Dot Blot Tutorial

website slot blot apparatus Slot Blot manifolds provide the ideal way to hybridise proteins and nucleic acids in solution onto membranes Easy to assemble, the slot blot can produce 48 slot images The western blot or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample

slot blot apparatus Add 200 ul of TBS to 48 chambers in the apparatus Apply the low-speed vacuum to filter the liquid through the membrane Add 200 ul of the fibril samples, the  Presoak 3 blotting papers and 1 nitrocellulose membrane in 6x SSC for 10 min · Clamp apparatus closed using the two  Vacuum-assisted dot blot apparatus has been used to facilitate the rinsing and incubating process by using vacuum to extract the solution

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